免费通用引物

引物名称 碱基数 序列
35S 20 mer 5'-GACGCACAATCCCACTATCC-3'
NOSR
18 mer 5'-AATCATCGCAAGACCGGC-3'
a-factor
21 mer  5'-TACTATTGCCAGCATTGCTGC-3'
3AOX1 21 mer
5'-GCAAATGGCATTCTGACATCC-3'
5AOX1 21 mer
5'-GACTGGTTCCAATTGACAAGC-3'
3AD 20 mer
5'-AGATGGTGCACGATGCACAG-3'
3DNA-BD 24 mer 5'-TTTTCGTTTTAAAACCTAAGAGTC-3'
3BD 25 mer 5'-TAAGAGTCACTTTAAAATTTGTATC-3'
5AD 21 mer 5'-AGGGATGTTTAATACCACTAC-3'
3GEX
23 mer  5'-CCGGGAGCTGCATGTGTCAGAGG-3'
5GEX 
23 mer  5'-GGGCTGGCAAGCCACGTTTGGTG-3'
EF1a-F
21 mer  5'-TCAAGCCTCAGACAGTGGTTC-3'
PCMV-F  
20 mer 5'-TCTAAAAGCTGCGGAATTGT-3'
PCMV-R   20 mer 5'-TCCAAACTCATCAATGTATC-3'
CMV-Forward
21mer 5'-CGCAAATGGGCGGTAGGCGTG-3'
CMV-R
16 mer 5'-GTTCACGGTGCCCTCC-3'
SV40-Promoter 19 mer 5'-GGAGGCTTTTTTGGAGGC -3'
EBV-Reverse 21 mer 5'-CAGCTTATAATGGTTACAAAT-3'
SV40pA-R 20 mer 5'-GAAATTTGTGATGCTATTGC-3'
BGHR 18 mer 5'-TAGAAGGCACAGTCGAGG-3'
hGH-PA-R 20 mer 5'-CCAGCTTGGTTCCCAATAGA-3'
M13(-96)
20 mer 5'-CCCTCATAGTTAGCGTAACG-3'
M13F(-47) 24 mer 5'-CGCCAGGGTTTTCCCAGTCACGAC-3'
M13F 18 mer 5'-TGTAAAACGACGGCCAGT-3'
M13R 17 mer 5'-CAGGAAACAGCTATGAC-3'
M13R(-48) 24 mer 5'-AGCGGATAACAATTTCACACAGGA-3'
pBABE-3 21 mer 5'-ACCCTAACTGACACACATTCC-3'
pBABE-5 17 mer  5'-CTTTATCCAGCCCTCAC-3'
pBAD-Forward 20 mer 5'-ATGCCATAGCATTTTTATCC-3'
pBAD-Reverse 18 mer 5'-GATTTAATCTGTATCAGG-3'
pBD-gal4-F 20 mer 5'-GCCTCTAACATTGAGACAGC-3'
pBD-gal4-R  22 mer 5'-AAGAGTTACTCAAGAACAAGAA-3'
PBV220F
20 mer 5'-AAGAAGGGCAGCATTCAAAG-3'
PBV220R 20 mer  5'-CTGCGTTCTGATTTAATCTG-3'
PTRC99C-F 18 mer 5'-TTGCGCCGACATCATAAC-3'
PEGFP-C-5  22 mer 5'-CATGGTCCTGCTGGAGTTCGTG-3'
PEGFP-C-3 20 mer  5'-TATGGCTGATTATGATCAGT-3'
PEGFP-N-5  22 mer  5'-TGGGAGGTCTATATAAGCAGAG-3'
PEGFP-N-3 22 mer 5'-CGTCGCCGTCCAGCTCGACCAG-3'
pFastBacF  20 mer  5'-GGATTATTCATACCGTCCCA-3'
pFastBacR  20 mer 5'-CAAATGTGGTATGGCTGATT-3'
p10-Promoter 19 mer 5'-CGGACCTTTAATTCAACCC-3'
pLXSN-F 20 mer 5'-CTCTCCCCCTTGAACCTCCT-3'
pLXSN-R 23 mer 5'-GAGCCTGGGGACTTTCCACACCC-3'
PMAL-C2X-F 20 mer 5'-TGCGTACTGCGGTGATCAAC-3'
PMAL-C2X-R 20 mer 5'-CTGCAAGGCGATTAAGTTGG-3'
PQE-F 20 mer 5'-TGAGCGGATAACAATTTCAC-3'
PQE-R 19 mer 5'-GTTCTGAGGTCATTACTGG-3'
RVP3 20 mer 5'-CTAGCAAAATAGGCTGTCCC-3'
RVP4 20 mer 5'-GACGATAGTCATGCCCCGCG-3'
GLP2 23 mer 5'-CTTTATGTTTTTGGCGTCTTCCA-3'
T3 20 mer 5'-ATTAACCCTCACTAAAGGGA-3'
T7  20 mer 5'-TAATACGACTCACTATAGGG-3'
T7-Term 19 mer  5'-GCTAGTTATTGCTCAGCGG-3'
pET-Up  20 mer 5'-GATCCCGCGAAATTAATACG-3'
DuetUp2 20 mer 5'-TTGTACACGGCCGCATAATC-3'
DuetDown1 20 mer  5'-GATTATGCGGCCGTGTACAA-3'
S-Tag  20 mer  5'-CGAACGCCAGCACATGGACA-3'
SP6  19 mer  5'-GATTTAGGTGACACTATAG-3'
pCold-F 19 mer  5'-ACGCCATATCGCCGAAAGG-3'
pCold-R 22 mer  5'-GGCAGGGATCTTAGATTCTGTG-3'
XL39
20 mer  5'-ATTAGGACAAGGCTGGTGGG-3'
V1_5  
18 mer 5'-GGACTTTCCAAAATGTCG-3'
ITS1 
19 mer  5'-TCCGTAGGTGAACCTGCGG-3'
ITS2 
21 mer  5'-GCTGCGTTCTTCATCGATGC -3'
ITS3 
21 mer  5'-GCATCGATGAAGAACGCAGC -3'
ITS4 
20 mer  5'-TCCTCCGCTTATTGATATGC-3'
ITS5
22 mer  5'-GGAAGTAAAAGTCGTAACAAGG-3'
27F 
20 mer 5'-AGAGTTTGATCCTGGCTCAG-3'
1492R 
19 mer 5'-GGTTACCTTGTTACGACTT-3'
PIRES3-EGFP-R 
20 mer 5'-AACGCACACCGGCCTTATTC-3'
PIRES-F 
20 mer  5'-CTTACTGACATCCACTTTGC-3'
PIRES-R  
20 mer 5'-CACTGCATTCTAGTTGTGGT-3'
Human-U6  18 mer  5'-GGGCAGGAAGAGGGCCTA-3'
LKO1-5
20mer
5'-GACTATCATATGCTTACCGT-3'
Mouse-U6
21mer
5'-CAGCACAAAAGGAAACTCACC-3'
pTT5-F
20mer
5'-CCGATCTGGCCATACACTTG-3'
pTT5-R
20mer
5'-TATGTCCTTCCGAGTGAGAG-3'

 

*您也可以下载金唯智引物与载体对照表,查看载体对应的通用引物。


金唯智通用引物选择工具

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使用说明:
(1)请输入您的目的载体或者需要查询的序列,点击查询,系统便会自动帮您选择测序通用引物。
(2)如果您的序列中含有数字或者空格,系统都会自动去除。