免费通用引物

 引物名称 序列
 35S 5'-GACGCACAATCCCACTATCC-3' 
NOSR  5'-AATCATCGCAAGACCGGC-3' 
a-factor   5'-TACTATTGCCAGCATTGCTGC-3'
 3AOX1 5'-GCAAATGGCATTCTGACATCC-3' 
5AOX1   5'-GACTGGTTCCAATTGACAAGC-3'
 3AD  5'-AGATGGTGCACGATGCACAG-3'
 3DNA-BD 5'-TTTTCGTTTTAAAACCTAAGAGTC-3' 
 3BD  5'- TAAGAGTCACTTTAAAATTTGTATAC -3'
5AD   5'-AGGGATGTTTAATACCACTAC-3'
 3GEX 5'-CCGGGAGCTGCATGTGTCAGAGG-3' 
5GEX   5'-GGGCTGGCAAGCCACGTTTGGTG-3' 
EF1a-F   5'-TCAAGCCTCAGACAGTGGTTC-3'
 PCMV-F    5'-TCTAAAAGCTGCGGAATTGT-3'
 PCMV-R   5'-TCCAAACTCATCAATGTATC-3'
CMV-Forward  5'-CGCAAATGGGCGGTAGGCGTG-3' 
CMV-R  5'-GTTCACGGTGCCCTCC-3' 
 SV40-Promoter 5'-GGAGGCTTTTTTGGAGGC -3' 
 EBV-Reverse  5'-CAGCTTATAATGGTTACAAAT-3'
 SV40pA-R  5'-GAAATTTGTGATGCTATTGC-3'
 BGHR  5'-TAGAAGGCACAGTCGAGG-3'
 hGH-PA-R  5'-CCAGCTTGGTTCCCAATAGA-3'
M13(-96)   5'-CCCTCATAGTTAGCGTAACG-3'
 M13F(-47)  5'-CGCCAGGGTTTTCCCAGTCACGAC-3'
 M13F  5'-TGTAAAACGACGGCCAGT-3'
 M13R  5'-CAGGAAACAGCTATGAC-3'
 M13R(-48)  5'-AGCGGATAACAATTTCACACAGGA-3'
 pBABE-3  5'-ACCCTAACTGACACACATTCC-3'
pBABE-5   5'-CTTTATCCAGCCCTCAC-3'
pBAD-Forward   5'-ATGCCATAGCATTTTTATCC-3'
pBAD-Reverse   5'-GATTTAATCTGTATCAGG-3'
pBD-gal4-F   5'-GCCTCTAACATTGAGACAGC-3'
pBD-gal4-R    5'-AAGAGTTACTCAAGAACAAGAA-3'
PBV220F   5'-AAGAAGGGCAGCATTCAAAG-3'
 PBV220R  5'-CTGCGTTCTGATTTAATCTG-3'
 PTRC99C-F  5'-TTGCGCCGACATCATAAC-3'
 PEGFP-C-5   5'-CATGGTCCTGCTGGAGTTCGTG-3'
 PEGFP-C-3  5'-TATGGCTGATTATGATCAGT-3'
PEGFP-N-5    5'-TGGGAGGTCTATATAAGCAGAG-3'
 PEGFP-N-3  5'-CGTCGCCGTCCAGCTCGACCAG-3'
pFastBacF    5'-GGATTATTCATACCGTCCCA-3'
 pFastBacR   5'-CAAATGTGGTATGGCTGATT-3'
p10-Promoter   5'-CGGACCTTTAATTCAACCC-3'
pLXSN-F   5'-CTCTCCCCCTTGAACCTCCT-3'
pLXSN-R   5'-GAGCCTGGGGACTTTCCACACCC-3'
 PMAL-C2X-F  5'-TGCGTACTGCGGTGATCAAC-3'
PMAL-C2X-R   5'-CTGCAAGGCGATTAAGTTGG-3'
PQE-F   5'-TGAGCGGATAACAATTTCAC-3'
 PQE-R  5'-GTTCTGAGGTCATTACTGG-3'
 RVP3  5'-CTAGCAAAATAGGCTGTCCC-3'
 RVP4  5'-GACGATAGTCATGCCCCGCG-3'
GLP2   5'-CTTTATGTTTTTGGCGTCTTCCA-3'
T3   5'-ATTAACCCTCACTAAAGGGA-3'
 T7   5'-TAATACGACTCACTATAGGG-3'
 T7-Term 5'-GCTAGTTATTGCTCAGCGG-3' 
pET-Up    5'-GATCCCGCGAAATTAATACG-3'
 DuetUp2  5'-TTGTACACGGCCGCATAATC-3'
 DuetDown1  5'-GATTATGCGGCCGTGTACAA-3'
S-Tag    5'-CGAACGCCAGCACATGGACA-3'
 SP6   5'-GATTTAGGTGACACTATAG-3'
pCold-F   5'-ACGCCATATCGCCGAAAGG-3'
 pCold-R  5'-GGCAGGGATCTTAGATTCTGTG-3'
 XL39  5'-ATTAGGACAAGGCTGGTGGG-3'
V1_5   5'-GGACTTTCCAAAATGTCG-3'
 ITS1   5'-TCCGTAGGTGAACCTGCGG-3'
 ITS2   5'-GCTGCGTTCTTCATCGATGC -3'
 ITS3   5'-GCATCGATGAAGAACGCAGC -3'
ITS4    5'-TCCTCCGCTTATTGATATGC-3'
 ITS5  5'-GGAAGTAAAAGTCGTAACAAGG-3'
27F   5'-AGAGTTTGATCCTGGCTCAG-3'
 1492R  5'-GGTTACCTTGTTACGACTT-3'
 PIRES3-EGFP-R   5'-AACGCACACCGGCCTTATTC-3'
 PIRES-F   5'-CTTACTGACATCCACTTTGC-3'
PIRES-R     5'-CACTGCATTCTAGTTGTGGT-3'
 Human-U6  5'-GGGCAGGAAGAGGGCCTA-3'
 LKO1-5  5'-GACTATCATATGCTTACCGT-3'
 Mouse-U6  5'-CAGCACAAAAGGAAACTCACC-3'
pTT5-F   5'-CCGATCTGGCCATACACTTG-3'
pTT5-R   5'-TATGTCCTTCCGAGTGAGAG-3'
 DuetUp1

 5'-GGATCTCGACGCTCTCCCT-3'

 PCDNA3-4-R  5'-CAACATAGTTAAGAATACCAGTC-3'
 HSVtk-pArev  5'-GTCTCCTTCCGTGTTTCAG-3'
 P1  5'-CCAGGCTTTACACTTTATGC-3'
 P2  5'-GCGATTAAGTTGGGTAACGC-3'
LZRS-F  5'-TGGATACACGCCGCCCACGTG-3' 
 LZRS-R  5'-ATCGTCGACCACTGTGCTGG-3'
 CMV-30  5'-ATAACCCCGCCCCGTTG-3'
CMV-24   5'-TTAGGACAAGGCTGGTGG-3'
 Ac5-Profor  5'-ACACAAAGCCGCTCCATCAG-3'
PH-Profor   5'-AAATGATAACCATCTCGC-3'
 pAcGP67rv  5'-TTGGTTCTTGCCGGGTC-3'
 5BD  5'-TCATCGGAAGAGAGTAG-3'
PB42ADF   5'-CCAGCCTCTTGCTGAGTGGAGATG-3'
PB42ADR   5'-AAGCCGACAACCTTGATTGGAG-3'
BAC1   5'-AACCATCTCGCAAATAAATA-3'
BAC2   5'-ACGCACAGAATCTAGCGCTT-3'
cIRep-Cfor   5'-TCCGTTGTGGGGAAAGTTATC-3'
 pBTrv  5'-GGGTAGCCAGCAGCATCC-3'
 pCAGGS-F  5'-TTCGGCTTCTGGCGTGTGAC-3'
 pCAGGS-R  5'-TATGTCCTTCCGAGTGAGAG -3'
 pDBLeu-F  5'-GAATAAGTGCGACATCATCATC-3'
 pDBLeu-R  5'-GTAAATTTCTGGCAAGGTAGAC-3'
 N26for  5'-CATCATAACGGTTCTGGC-3'
 pTrcHis-rv  5'-ATCTGTATCAGGCTGAAAATC-3'
 malE  5'-GGTCGTCAGACTGTCGATGAAGCC-3'
 pMAL-C5x-R  5'-TGTCCTACTCAGGAGAGCGTTCAC-3'
 PJET1-2-F  5'-CGACTCACTATAGGGAGAGCGGC-3'
 PJET1-2-R 5'-AAGAACATCGATTTTCCATGGCAG-3' 
 pDsRED-ex-C1-F  5'-TCCCACAACGAGGACTACAC-3'
pDSRED-N1-R   5'-TGAAGCGCATGAACTCCTTG-3'
pYes2-R   5'-GCGTGAATGTAAGCGTGAC-3'
PLEXA-F   5'-CGTCAGCAGAGCTTCACCATTG-3'
PLEXA-R   5'-TAAAACCTAAGAGTCACTTT-3'
 pLNCX-F  5'-AGCTCGTTTAGTGAACCGTCAGATCG-3'
 PLNCX-R  5'-ACCTACAGGTGGGGTCTTTCATTCCC-3'
NL1   5'-GCATATCAATAAGCGGAGGAAAAG-3'
 NL4  5'-GGTCCGTGTTTCAAGACGG-3'
 H1 5'-TCGCTATGTGTTCTGGGAAA-3' 
V5-R   5'-ATCGAGACCGAGGAGAGG-3'
 GLp1 5'-TGTATCTTATGGTACTGTAACTG-3' 
MSCV-Rev   5'-CAGCGGGGCTGCTAAAGCGCATGC-3'
AMP-F   5'-GGTCCTCCGATCGTTGTCAG-3'
 AMP-R  5'-GACGCCGGGCAAGAGCAACT-3'
 KANA-F  5'-GACGAGGGGAAATTAATAGGTTGT-3'
 KANA-R  5'-CACCTGATTGCCCGACATTAT-3'
       

 

*您也可以下载安升达引物与载体对照表,查看载体对应的通用引物。

 



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